MiR-449a regulates caprine endometrial stromal cell apoptosis and endometrial receptivity.
In this study, RT-qPCR analysis showed that the expression level of miR-449a in the pre-receptive endometrium were lower than receptive endometrium, Rabbit Clia Kits which is consistent with previous sequencing data (the previous investigation). To detect the role of miR-449a in endometrial receptivity, we transfected caprine endometrium stromal cells (ESCs) cultured in vitro with miR-449a mimic. The results showed that miR-449a to decrease the mRNA and protein level by targeting LGR4 3'-translated region of her. MiR-449a mimic significantly reduce G1 cell population of 52.56% (mimic NC) to 42.19% with a corresponding increase in the G2 and S-cell population of 47.44% (mimic NC) to 57.81%, indicating that miR-449a caused cell cycle arrest ESC. In addition, the number of apoptotic cells was significantly higher in ESCs transfected with miR-449a mimic (P <0.05) compared to ESCs transfected by imitating NC. In vivo, rich pinopodes observed on the surface of the endom...