Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases.

DNA methylation plays an important role in reproduction. Whole genome DNA methylation changes during oestrous phase (ES) and phase dioestrous (DS) in the ovaries Guanzhong dairy goats were investigated using bisulfite sequencing to understand the molecular biological mechanism of this goat oestrous cycle.

result
We found a pattern of genome-wide DNA methylation in ES and DS differ ovaries. As many as 26 910 different methylated region that is upregulated and 21 453 Rat Clia Kits different methylated region that is downregulated in ES samples compared with samples DS (P-value ≤0.05 and fold change of methylation ratios ≥2). Analysis of different regions showed hypomethylation methylated genes in the body and hypermethylation in the join between the upstream region and gene bodies. 

Methylation ratio of STAR, FGF2, FGF12, BMP5 and SMAD6 genes in ES samples is lower than the DS samples (P-value ≤0.05 and fold change of methylation ratios ≥2). By contrast, the ratio of the EGFR gene methylation, TGFBR2, IGF2BP1 and MMD2 increased in ES samples compared with samples DS. In addition, 223 genes vary methylated found in GnRH signaling pathway (KO04912), ovarian steroidogenesis pathways (KO04913), estrogen signaling pathway (KO04915), oxytocin signaling pathway (KO04921), insulin secretion pathway (KO04911) and MAPK signaling pathway ( KO04010).
conclusion

This study is the first large-scale comparison of high-resolution DNA methylation landscape ovarian oestrous and dioestrous from goat's milk. previous studies and our investigations have shown that NR5A2, STAR, FGF2 and BMP5 genes may have potential application value in regulating oestrus caprine.
Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases.


Mycoplasma capricolum subsp. capripneumoniae, the cause of the World Organization registered with the animal health contagious caprine pleuropneumonia, is a member of the Mycoplasma mycoides cluster consists of five pathogenic mycoplasmas of ruminants. mycoplasmas closely related immunological and genetic that can cause difficulties for differential diagnosis. 

The pattern of substrate metabolism of the strains of M. c. capripneumoniae, collected from diverse geographic regions, studied by measuring the rate of Simian Clia Kits oxygen uptake. Strain fall into two main biochemical groups: one that only oxidized organic acids and glycerol and others that can also metabolize sugar. 

Furthermore, when the DNA-DNA hybridization tests performed both groups strains may be separated by their degree of DNA homology, the average value of hybridization between members of both groups was 86% above the value of 70% is usually used to indicate a separate species. DNA-DNA hybridization is also carried out between AD c. capripneumoniae strains and other members of the M. mycoides cluster.

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