Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter.

Related keratin protein (KAP) is a structural component of cashmere fibers, and variations in several genes KAP (KRTAPs) has been associated with a number of caprine fiber properties. In this study, we report the identification KRTAP15-1 Equine Clia Kitsin goats. gene sequence variations are detected using the polymerase chain reaction single-strand conformation polymorphism technique (PCR-SSCP) in 250 goats Longdong, and six variants (named A to F) contains eight single nucleotide polymorphisms (SNPs) have been identified. Five of the SNPs are non-identical and will cause the suspected amino acid changes. 

Reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that KRTAP15-1 secondary expressed in hair follicles but not in heart tissue, liver tissue, lung tissue, kidney tissue or Longissimus dorsi muscle. Although rich in cysteine, which caprine KAP15-1 protein has a high content of serine and glycine and phenylalanine moderate content. 

Association analysis revealed that KRTAP15-1 variant A was associated with a decrease in the average fiber diameter (MFD), and this effect appears to be dominant; while the C variant found to be associated with increased MFD, the effects are recessive. The findings show that caprine KRTAP15-1 highly polymorphic and that variations in this gene affect cashmere MFD.
Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter.


Caprine anaplasmosis is a tick-borne rickettsial disease that affects goats economically important throughout the world. Microscopic examination of stained blood smears from 162 animals revealed inclusion bodies of Anaplasma spp. in 24 cases. Genus specific PCR for Anaplasma spp. yielded positive results in 22 cases. All sick animals showed a significant decrease in the average value of antioxidants such as reduced glutathione, superoxide dismutase and catalase and a significant increase in the level of lipid peroxidation. 

Of the 22 animals positive for General Clia Kits anaplasmosis both in the blood smear and PCR, 16 non-pregnant goats were selected for the study women were divided into two groups of eight animals each. Animals including group I and II were treated with oxytetracycline dihydrate. 

Moreover, animals of group II are equipped with a combination of vitamin E-selenium. oxidative stress parameters were rechecked at day 10 of treatment. At the end of the study period, a significant decrease in malondialdehyde levels and a significant increase in the average value of superoxide dismutase was detected in Group II.

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